reverse transcription pcr

Detection of Hepatitis A Virus by Two Reverse Transcription PCR Approaches

两种反转录聚合酶链反应检测甲型肝炎病毒

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Methods: The putative envelope protein 2 gene fragments of HCV in sera from Shandong ( SD), Shanghai ( SH) patients were amplified by reverse transcription nested PCR and sequenced.

方法：对来自山东（SD）、上海（SH）两地患者血清RNA进行逆转录－巢式PCR，扩增HCV囊膜蛋白2基因片段，用PCR直接测序法对该片段进行序列测定。

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Real-time fluorescence quantitative reverse transcription PCR in determination of guanylyl cyclase-C gene expression in peripheral blood of patients with colorectal cancer and its clinical significance

实时荧光定量PCR检测大肠癌患者外周血鸟苷酰环化酶C基因及其临床意义

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Diagnosis of Infectious Bursal Disease Virus by Reverse Transcription Nested PCR

反转录&套式PCR方法检测鸡传染性法氏囊病病毒

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Reverse transcription PCR and in situ hybridization were used to detect the expression changes of CaBP mRNA in the brain of PD and control mice.

用反转录PCR及原位分子杂交方法检测PD鼠和正常鼠脑内CaBP mRNA的表达变化。

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The silencing effects of TIMP-1 on the mRNA and protein levels were examined by reverse transcription PCR ( RTPCR) and Western blot analysis respectively.

采用RT-PCR和Western印迹测定宿主细胞TIMP-1在基因和蛋白水平的沉默效果。

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The rats of group B were treated with EPO after globe ischemia-reperfusion. Brain tissues were taken out after execution at different time point. The expression of caspase-9 mRNA was detected with reverse transcription PCR ( RT-PCR) technique.

在相应时间点断头取脑，逆转录聚合酶链反应技术检测大鼠皮质caspase9mRNA的表达，并与对照组（C组）比较。

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A Reverse Transcription PCR that Distinguishes the Isolates of Low Virulent Strains From Virulent Strains of Newcastle Disease Virus

鸡新城疫病毒强弱毒株RT-PCR鉴别诊断方法

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Then, RT-PCR was carried out for IL-10 gene screening using total RNA as a template, specific primers which derived from GenBank data of hIL-10 as a primer for reverse transcription and PCR primers.

以总RNA为模板，根据人IL-10在GenBank中的全长开放读框设计的特异性引物进行反转录，RT-PCR进行IL-10基因筛选。PCR产物经酶切分析和DNA序列测定后；

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CDNA-AFLP differential patterns for 5 selected genes were confirmed via real-time reverse transcription PCR analysis.

用荧光实时定量PCR分析法证实了cDNA-AFLP基因表达结果。

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A reverse transcription PCR system was established for the detection of Noroviruses in shellfish.

本文建立了贝类产品中诺沃克病毒检测的普通RT-PCR方法。

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Application of reverse transcription PCR in the detection of respiratory syncytial virus infection

反转录-聚合酶链反应在呼吸道合胞病毒感染检测的应用

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Methods The interested segment was obtained by reverse transcription PCR with the designed specific primers, and inserted into pMD-18T vector by T/ A match.

方法设计特异引物，用RT-PCR方法从乳腺癌组织扩增获得目的片段，利用T/A克隆将PCR产物插入pMD-18T载体；

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Methods Techniques of cell culture in vitro and RT PCR ( reverse transcription PCR) were used, gene expressions of p53 and fas in 4 tumor cell lines were measured after treated with ethanol extractive and water extractive.

方法采用肿瘤细胞体外培养技术及RT-PCR技术，观察阿魏菇不同剂量的水提物及醇提物对体外培养的四种肿瘤细胞p53及fas基因表达的影响。

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Methods: Total RNA were extracted from human peripheral blood, and reverse transcription PCR ( RT PCR) were used to amplify the whole gene sequences of HLA DR loci, and the PCR products were linked to vector and transformed to E.

方法：提取人外周血总RNA，RT PCR扩增HLA DR等位基因全序，连接、转化大肠杆菌后并测序。

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Reverse transcription PCR analyses show that the goose PRLR mRNA is widely expressed in testis, seminal duct, ovary, oviduct, kidney, large intestine, and small intestine and the highest abundance are presented in kidney, oviduct, large intestine and small intestine.

RT-PCR结果表明，鹅PrlR mRNA在成年鹅睾丸、输精管、卵巢、输卵管、肾、大肠、小肠、脾组织中均有表达，其中以肾、睾丸、大肠及小肠中表达最为丰富。

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We primarily studied the differential expressions of arabidopsis thaliana seedlings under sound stimulation by mRNA reverse transcription differential display PCR ( DDRT-PCR) with silver staining in this thesis.

本文运用银染mRNA逆转录差异显示（DDRT-PCR）技术，初步研究了声波刺激对拟南芥基因表达差异的影响。

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Consequently, the positive ESTs which significantly correlate with lipidosis trait of goose were screened by Delta Differential Display Reverse Transcription PCR ( DDRT-PCR) method and the high homologue relative genes were found by cloning, sequencing and homology analysis.

采用δ差异显示法筛选出与鹅脂肪沉积性状显著相关的阳性EST，进行了克隆测序和同源性比对分析，找出了高度同源性的相关基因。

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Rapid identification of subtypes of respiratory syncytial virus by real-time reverse transcription PCR

实时逆转录聚合酶链反应快速鉴别呼吸道合胞病毒亚型

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An one step procedure was developed using the PCR ( polymerase chain reaction) for the detection of both hepatitis A virus and poliovirus RNA, in which reverse transcription and PCR were processed in one tube.

建立的一步PCR方法即反转录和PCR在同一管中进行，同时检测甲型肝炎和脊髓灰质炎病毒病毒RNA。

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Identification of Viruses Causing Respiratory Tract Infection by Single-tube Multiplex Reverse Transcription PCR

应用多重反转录PCR技术检测病毒性呼吸道感染

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Method: The cardiac tissue was taken from 30 cadavers of CHD and 20 cadavers of non CHD. Primers were derived from RV gene encoding sequence of the enveloped protein E 1 which was highly conserved in rubella virus by reverse transcription PCR in cardiac tissue.

方法：以RV特异的糖蛋白E1编码的基因保守序列设计引物，采用RT-PCR技术，对30例CHD及20例非先天畸形（对照组）尸体的心肌组织标本进行RV基因的检测。

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A coupled one-step reverse transcription PCR procedure for cloning the human augmenter of liver regeneration gene

RT-PCR一步法克隆人肝再生增强因子基因

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Clone of EPO Gene from Human Fetal Liver cDNA by Reverse Transcription and PCR

人胎肝cDNA中EPO基因的克隆

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Application of in situ reverse transcription PCR techniques

原位反转录PCR技术的应用

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Methods Total RNA was extracted from peripheral blood lymphocytes ( PBL) of patients with glioma and subjected to reverse transcription PCR. Variable region genes ( Vk and VH gene) were amplified from the cDNA by using nested polymerase chain reaction.

方法从脑胶质瘤患者外周血淋巴细胞（PBL）中提取细胞总RNA，经逆转录后用套式多聚酶链反应（PCR）分别扩增抗体轻链VK和重链VH基因。

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The method of reverse transcription PCR ( RT-PCR) is more sensitive and reliable than that of enzyme-linked immunosorbent assay on nitrocellulose membranes ( NCM-ELISA).

对于甘薯病毒病的检测，反转录PCR法（RT-PCR）比硝化纤维素膜酶联免疫吸附检测法（NCM-ELISA）更灵敏和可靠。

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Recombinant human EGF was used to stimulate HepG_2 cells and semi-quantitative reverse transcription PCR was adopted to detect the expression of VEGF in HepG_2 cells.

离体实验中，用重组人EGF刺激人肝癌细胞系HepG2，采用半定量逆转录PCR检测VEGF的表达情况。

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The gene expression of a-actin mRNA were determined by using the quantitative reverse transcription PCR technology.

利用半定量反转录聚合酶链式反应(QRT-PCR)法测定骨骼肌α-actin基因表达.

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Methods Both real-time fluorescence quantitative RT-PCR and reverse transcription specific primers PCR was applied to analyze serum HCV RNA copy numbers and genotypes.

方法用实时荧光定量RT PCR和逆转录型特异性引物PCR同时检测21例费城酒精依赖丙肝患者的血清HCV RNA拷贝数并分析HCV基因型。

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